Actin dynamics regulate proteasome homeostasis

Cells require thousands of unique proteins to be in the right place, at time, amounts and with modifications. They do this through several processes collectively known as protein homeostasis. TORC1 is a principal regulator homeostasis, coordinating synthesis degradation. The proteasome, composed core particle one or two regulatory particles, degrades unwanted protein. Diverse stresses cause homeostasis imbalance: inhibiting misfolded/damaged load. Following inhibition, proteasome assembly chaperone (RPAC) translation increased thus cells assemble more proteasomes degrade damaged misfolded proteins, thereby restoring Using yeast, we identify an endocytic protein, Ede1, that interacts critical for RPAC mRNA following inhibition. We find further important regulation. Mutants these altered Arp2/3 activity, hence formation actin patches/endocytic sites. show transported on cables patches. inhibition depolarises cytoskeleton, causing accumulation patches concurrent translation. demonstrate Ede1 essential localisation regulation rapamycin treatment. This work shows that, upon depolarisation, recruited patches, likely by occurs. Actin therefore key element (and protein)